Thermo Fisher Scientific Human PDGF-BB Recombinant Protein
인간 PDGF-BB 재조합 단백질로, 세포 성장 및 분화 연구에 적합합니다. E. coli 발현 시스템에서 생산되며, 순도 95% 이상입니다. 기능적 시험에서 높은 생물학적 활성을 나타내며, 보존제 무첨가 상태로 제공됩니다.
- 카탈로그번호
- PHG004x (5개 옵션)
- 카테고리
- Protein
- 판매단위
- pk
카탈로그
5개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Human PDGF-BB Recombinant Protein
Thermo Fisher Scientific Human PDGF-BB Recombinant Protein
Applications
- Western Blot (WB): Assay-dependent
- Immunohistochemistry (IHC): Assay-dependent
- ELISA: Assay-dependent
- Functional Assay: Assay-dependent
- Control (Ctrl): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | E. coli |
| Amino Acid Sequence | SLGSLTIAEP AMIAECKTRT EVFEISRRLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RPTQVQLRPV QVRKIEIVRK KPIFKKATVT LEDHLACKCE TVAAARPVT |
| Molecular Weight | 25.4 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | >95% by SDS-PAGE |
| Endotoxin Concentration | <0.1 ng/µg |
| Activity | ED50 ≤ 5 ng/mL; determined by the dose-dependent proliferation of BALB/c 3T3 cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
Carrier-Free
Reconstitution:
Centrifuge the vial briefly before opening to bring the contents to the bottom. Reconstitute the lyophilized protein in 100 mM acetic acid containing 0.1% BSA to 0.1–1.0 mg/mL to regain full activity. Apportion the reconstituted protein into working aliquots and store at ≤ -20°C. Make further dilutions in low endotoxin medium or buffered solution with FBS or tissue culture grade BSA.
Storage:
Store the lyophilized protein at 2–8°C, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at ≤ -20°C. Avoid repeated freeze-thaw cycles.
Target Information
The protein encoded by this gene is a member of the platelet-derived growth factor (PDGF) family. These factors are mitogenic for cells of mesenchymal origin and characterized by a motif of eight cysteines.
PDGF-BB can exist as a homodimer or as a heterodimer with PDGF-AB, connected by disulfide bonds.
Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, involving COL1A1, are linked to dermatofibrosarcoma protuberans, resulting from unregulated growth factor expression.
Two alternatively spliced transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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