Thermo Fisher Scientific Human PDGF-BB Recombinant Protein

Thermo Fisher Scientific Human PDGF-BB Recombinant Protein

Applications

• Western Blot (WB): Assay-dependent
• Immunohistochemistry (IHC): Assay-dependent
• ELISA: Assay-dependent
• Functional Assay: Assay-dependent
• Control (Ctrl): Assay-dependent

Product Specifications

Product Specific Information

Carrier-Free

Reconstitution:
Centrifuge the vial briefly before opening to bring the contents to the bottom. Reconstitute the lyophilized protein in 100 mM acetic acid containing 0.1% BSA to 0.1–1.0 mg/mL to regain full activity. Apportion the reconstituted protein into working aliquots and store at ≤ -20°C. Make further dilutions in low endotoxin medium or buffered solution with FBS or tissue culture grade BSA.

Storage:
Store the lyophilized protein at 2–8°C, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at ≤ -20°C. Avoid repeated freeze-thaw cycles.

Target Information

The protein encoded by this gene is a member of the platelet-derived growth factor (PDGF) family. These factors are mitogenic for cells of mesenchymal origin and characterized by a motif of eight cysteines.
PDGF-BB can exist as a homodimer or as a heterodimer with PDGF-AB, connected by disulfide bonds.
Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, involving COL1A1, are linked to dermatofibrosarcoma protuberans, resulting from unregulated growth factor expression.
Two alternatively spliced transcript variants encoding different isoforms have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.