Thermo Fisher Scientific Human IGF-II Recombinant Protein
인간 IGF-II 재조합 단백질로 세포 증식 및 생존 연구에 활용 가능. E. coli 발현 시스템에서 생산되며, ≥98% 고순도. 기능적 활성 검증 완료(ED50 ≤ 2 ng/mL). 장기 보관 시 -20°C 이하에서 안정적 유지.
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- Protein
- 판매단위
- pk
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Thermo Fisher Scientific Human IGF-II Recombinant Protein
Applications
- Western Blot (WB): Assay-dependent
- Immunohistochemistry (IHC): Assay-dependent
- ELISA: Assay-dependent
- Functional Assay: Assay-dependent
- Control (Ctrl): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | E. coli |
| Amino Acid Sequence | AYRPSETLCG GELVDTLQFV CGDRGFYFSR PASRVSRRSR GIVEECCFRS CDLALLETYC ATPAKSE |
| Molecular Weight | 7.5 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | ≥98% by SDS-PAGE and HPLC |
| Endotoxin Concentration | <0.1 ng/µg |
| Activity | ED50 ≤ 2 ng/mL; determined by dose-dependent proliferation of FDC-P1 cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice (domestic); Wet or Dry ice (international) |
Product Specific Information
Reconstitution:
Briefly centrifuge vial before opening. Reconstitute in sterile distilled water to 0.1–1.0 mg/mL. Do not vortex.
Store reconstituted solution at 2–8°C for up to 1 week. For long-term storage, dilute with buffer containing 0.1% BSA and store aliquots at -20°C to -80°C.Storage:
Lyophilized protein stable at room temperature for 1 month; for long-term, store at -20°C, preferably desiccated.
Working aliquots with carrier protein stable ≥12 months at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
Target Information
IGFs are mitogenic polypeptide growth factors that promote proliferation and survival of various cell types, including muscle, bone, and cartilage in vitro.
Produced mainly by the liver, IGFs belong to the insulin gene family, sharing structural and functional similarity with insulin but exhibiting stronger growth-promoting activity.
IGF-II expression is influenced by placental lactogen, while IGF-I is regulated by growth hormone. Both signal through IGF-IR, though IGF-II can also interact with the IGF-II/Mannose-6-phosphate receptor. Mature IGFs are formed by proteolytic processing of inactive precursors containing N- and C-terminal propeptides.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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