Thermo Fisher Scientific SARS-CoV-2 Spike Protein S1 (aa14-683), His-Avi Tag Recombinant Protein

Applications

• ELISA: Assay-dependent
• Functional Assay: Assay-dependent
• Control: Assay-dependent
• Surface Plasmon Resonance (SPR): Assay-dependent

Product Specifications

Activity

Measured by its binding ability in a functional ELISA.

• Immobilized Recombinant SARS-CoV-2 Spike S1 at 2 µg/mL (100 µL/well) can bind recombinant Human ACE2 with a linear range of 0.15–6.85 ng/mL.
• Immobilized Human ACE2 on COOH Chip can bind SARS-CoV-2 Spike S1 with an affinity constant of 24.5 nM as determined in an SPR assay (Nicoya OpenSPR).

Product Specific Information

Reconstitution:
Reconstitute to a concentration of 0.1–0.5 mg/mL in sterile distilled water.
After reconstitution, the protein solution is stable at -20°C for 3 months, or at 2–8°C for up to 1 week.

Target Information

SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2), also known as 2019-nCoV, is a virus that causes illnesses ranging from mild cold-like symptoms to severe respiratory distress and death.
The Spike Protein is composed of S1 and S2 domains and serves as the main surface antigen of the coronavirus. The S1 domain contains a receptor-binding domain (RBD) that specifically binds to angiotensin-converting enzyme 2 (ACE2) on target cells.
The glycosylated Spike protein can be detected in infected cell culture supernatants with antisera from SARS patients. Variations in the Spike protein reflect CoV diversity and influence receptor interactions and virus-cell membrane fusion mechanisms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.