Thermo Fisher Scientific SARS-CoV-2 Spike Protein S1 (aa11-682), hFc-His Tag Recombinant Protein

Thermo Fisher Scientific SARS-CoV-2 Spike Protein S1 (aa11-682), hFc-His Tag Recombinant Protein

Applications

• Western Blot (WB): Assay-dependent
• ELISA: Assay-dependent
• Functional Assay: Assay-dependent
• Control (Ctrl): Assay-dependent
• Surface Plasmon Resonance (SPR): Assay-dependent

Product Specifications

Activity

• Measured by its binding ability in a functional ELISA.
  • Immobilized Recombinant Human ACE2 at 2 µg/mL (100 µL/well) can bind Recombinant SARS-CoV-2 Spike S1, ED50 of SARS-CoV-2 Spike S1 is 6.79 ng/mL.
  • Immobilized Human ACE2 on COOH Chip can bind SARS-CoV-2 Spike S1 with an affinity constant of 90.8 nM (SPR assay, Nicoya OpenSPR).

Product Specific Information

• Reconstitution: Reconstitute to a concentration of 0.1–0.5 mg/mL in sterile distilled water.
  After reconstitution, the protein solution is stable at -20°C for 3 months, at 2–8°C for up to 1 week.
• Description: Recombinant 2019-nCoV Spike S1 Protein produced by HEK293 cell expression system.

Target Information

SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2), also known as 2019-nCoV, causes illnesses from mild cold symptoms to severe respiratory distress and death.
The Spike Protein consists of S1 and S2 domains, serving as the main surface antigen.
S1 contains the receptor-binding domain (RBD) that binds specifically to ACE2 on target cells.
Spike protein diversity reflects variations in receptor interactions and responses to environmental triggers of virus-cell membrane fusion.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.