Thermo Fisher Scientific SARS-CoV-2 Spike Protein S1 (aa11-682), hFc-His Tag Recombinant Protein
SARS-CoV-2 Spike Protein S1 (aa11-682) 재조합 단백질로, HEK293 세포에서 발현된 Fc-His 태그 단백질입니다. ELISA, WB, SPR 등 다양한 분석에 활용 가능하며, ACE2 결합 활성 검증됨. 고순도(>90%) 및 저엔도톡신(<0.1 EU/µg) 특성으로 연구용에 적합.
- 카탈로그번호
- RP87679
- 카테고리
- Protein
- 판매단위
- pk
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Thermo Fisher Scientific SARS-CoV-2 Spike Protein S1 (aa11-682), hFc-His Tag Recombinant Protein
Applications
- Western Blot (WB): Assay-dependent
- ELISA: Assay-dependent
- Functional Assay: Assay-dependent
- Control (Ctrl): Assay-dependent
- Surface Plasmon Resonance (SPR): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Virus |
| Expression System | HEK293 cells |
| Amino Acid Sequence | SARS-CoV-2 Spike Protein S1, amino acids Val11-Arg682 (Accession # YP_009724390.1) with a C-terminal human Fc-tag and His-tag |
| Tag | Fc-tag, His-tag |
| Molecular Weight | 103 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | >90% by SDS-PAGE |
| Endotoxin Concentration | <0.1 EU/µg |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Wet ice |
Activity
- Measured by its binding ability in a functional ELISA.
- Immobilized Recombinant Human ACE2 at 2 µg/mL (100 µL/well) can bind Recombinant SARS-CoV-2 Spike S1, ED50 of SARS-CoV-2 Spike S1 is 6.79 ng/mL.
- Immobilized Human ACE2 on COOH Chip can bind SARS-CoV-2 Spike S1 with an affinity constant of 90.8 nM (SPR assay, Nicoya OpenSPR).
Product Specific Information
- Reconstitution: Reconstitute to a concentration of 0.1–0.5 mg/mL in sterile distilled water.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2–8°C for up to 1 week. - Description: Recombinant 2019-nCoV Spike S1 Protein produced by HEK293 cell expression system.
Target Information
SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2), also known as 2019-nCoV, causes illnesses from mild cold symptoms to severe respiratory distress and death.
The Spike Protein consists of S1 and S2 domains, serving as the main surface antigen.
S1 contains the receptor-binding domain (RBD) that binds specifically to ACE2 on target cells.
Spike protein diversity reflects variations in receptor interactions and responses to environmental triggers of virus-cell membrane fusion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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