Thermo Fisher Scientific MEK2 N-Term Monoclonal Antibody (19G10.F1.E2), Biotin
MEK2 N-말단을 인식하는 Biotin 결합 단일클론 항체로, Human, Mouse, Rat에 반응. Western blot 및 ELISA에 적합하며 Protein A로 정제된 고순도 제품. 연구용으로 MEK2 신호전달 경로 분석에 활용 가능.
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Thermo Fisher Scientific MEK2 N-Term Monoclonal Antibody (19G10.F1.E2), Biotin
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| ELISA | 1:40,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 19G10.F1.E2 |
| Immunogen | Synthetic peptide corresponding to amino acid residues near the N-terminus conjugated to KLH |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
- Unconjugated
- DyLight 680
- DyLight 800
- FITC
- HRP
- Custom conjugation available upon request
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid freeze/thaw cycles. Centrifuge product if not completely clear after standing at room temperature.
Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Reacts specifically with human MEK2. Cross-reactivity is expected with human, mouse, and rat based on sequence identity of the peptide immunogen. This antibody does not react with the MEK1 isoform.
Target Information
MAP2K2 (MEK2) is a dual specificity serine/threonine kinase involved in mitogen growth factor signal transduction. MEK2 (MAPKK2) activates ERK1/2 and MAPK enzymes by phosphorylating residues within the TEY motif in the activation loop.
MEK1 and MEK2 are activated by dual phosphorylation on serine 218 and 222, and threonine 292 of MEK1 is phosphorylated by ERK2, forming a negative feedback loop that suppresses MEK1 activation.
Usage Notice
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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