Thermo Fisher Scientific Phospho-AKT (Thr308) Monoclonal Antibody (18F3.H11), Biotin
Phospho-AKT (Thr308) 단백질을 특이적으로 인식하는 Biotin 결합 단클론 항체. Western blot, IHC, ELISA, IP에 적합. 인간, 마우스 등 다양한 종에 반응. 암, 신호전달 및 신경과학 연구용으로 최적화. 장기보관 시 -20°C 권장.
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Thermo Fisher Scientific Phospho-AKT (Thr308) Monoclonal Antibody (18F3.H11), Biotin
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 20 µg/mL |
| ELISA | 1:20,000 |
| Immunoprecipitation (IP) | 1:500–1:3,000 |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 18F3.H11 |
| Immunogen | Synthetic peptide corresponding to residues surrounding T308 of human AKT1 protein |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate (pH 7.2), 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C (short term); -20°C (long term), avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot and freeze at -20°C or below.
- Avoid freeze/thaw cycles.
- Centrifuge if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as undiluted liquid.
- Dilute only prior to immediate use.
This antibody specifically recognizes human and mouse AKT protein phosphorylated at T308.
BLAST analysis suggests cross-reactivity with AKT pT308 from most vertebrates based on 100% sequence homology.
Cross-reactivity with AKT2 and AKT3 is likely.
Recommended for Cancer, Cell Signaling, Neuroscience, and Signal Transduction research.
Target Information
AKT1 (PKB alpha) is a serine/threonine kinase regulating cell survival.
Activated AKT1 inhibits apoptosis and promotes cell cycle progression by phosphorylating multiple targets, including forkhead transcription factors and caspase-9.
Activation occurs via insulin, PI3K, IGF1, and other growth factors.
The AKT pathway is a major focus in cancer drug discovery.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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