Thermo Fisher Scientific FOXP3 Polyclonal Antibody, HRP
FOXP3 단백질을 인식하는 HRP 결합 폴리클로날 항체로, Western blot 및 IHC(P/F)에 적합. 인간 및 마우스 시료에 반응하며 항원 친화 크로마토그래피로 정제됨. 4°C 암소 보관, 연구용 전용 제품.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:50 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to the C-terminal region of human FOXP3 (residues 400–431) |
| Conjugate | HRP |
| Form | Liquid |
| Concentration | 0.88 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Wet ice |
| RRID | AB_11152573 |
Product Specific Information
This antibody does not detect mouse FOXP3 in Western blot but does detect mouse tissue for IHC.
Target Information
FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals and essential for normal immune homeostasis. It is 381 amino acids long, expressed at high levels in CD25⁺CD4⁺ regulatory T cells, low levels in CD4⁺/CD25⁻ cells, and absent in CD4⁻/CD8⁺ T cells. FOXP3 acts as a master regulatory gene and a specific marker of regulatory T cells. Mutations in FOXP3 cause the scurfy phenotype in mice and IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked) in humans. Expression of FOXP3 in human breast and colon cancer cells is regulated by p53 in response to DNA damage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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