Merck Anti-Collagen, Type X antibody, Mouse monoclonal

Anti-Collagen, Type X antibody, Mouse monoclonal

clone COL-10, purified from hybridoma cell culture

Anti-COL10A1

생물학적 소스

mouse

항체 형태

purified from hybridoma cell culture

antibody product type

primary antibodies

클론

COL-10, monoclonal

form

buffered aqueous solution

분자량

~60 kDa

species reactivity

deer, porcine, human

포장

antibody small pack of 25 μL

농도

~1 mg/mL

technique(s)

immunoblotting: suitable
immunofluorescence: 5-10 μg/mL using human osteosarcoma SaOS-2 cells
immunohistochemistry: suitable

동형

IgM

UniProt 수납 번호

Q03692

배송 상태

dry ice

저장 온도

−20°C

The extracellular matrix (ECM) found in the extracellular environment of all tissues and organs, provides the physical microenvironment for cells and a substrate for cell anchorage. It serves as a tissue scaffold and is a dynamic structure whose organization and composition modulate various cellular processes including cell proliferation, attachment, migration, differentiation and survival. The composition of the extracellular framework of all vertebrates is dominated by a Collagen protein family, each member with unique features suited for its function and location.

Type X collagen,also known as Collagen alpha-1(X) chain (COL10A1), is a product of hypertrophic chondrocytes. It shares a similar domain structure with type VIII collagen. In addition, both collagen types represent major components of hexagonal lattice structure, in which the collagen molecules link together by interactions involving the non-triple-helical end regions. Despite these similarities, a distinct tissue distribution has been found for these two molecules: type VIII collagen is distributed in various tissues, whereas type X is restricted to normal fetal hypertrophic cartilage in the growth zones of long bones, vertebrae and ribs and in adult (> 21 yr) thyroid cartilage. It is also found in bone fracture callus, osteoarthritic cartilage and chondrogenic neoplasms, and may be involved in cartilage mineralization. Type X collagen is non-fibrillar, but forms fine pericellular filaments in association with cartilage collagen. It interacts with matrix proteins, such as connexin V, chondrocalcein, collagen II and proteoglycans, as well as with Ca2+ . Mutations in this gene are associated with schmid metaphyseal chondroplasia (MCDS).

The development of antibodies against collagens has provided a powerful method for examining the distribution of these connective tissue proteins and for investigation of epithelial-mesenchymal interactions, tumorigenesis and basement membrane biology in ontogeny and epithelial differentiation.8 Antibodies that react specifically with collagen type X are useful for the study of specific differential tissue expression and the localization of collagen type X.