Merck Dispase II

Dispase® II

protease

Protease from Bacillus polymyxa

Quality Level

100

form

lyophilized solid

specific activity

≥0.5 units/mg solid

solubility

10 mM NaAc (pH 7.5) and 5 mM CaAc: soluble

저장 온도

2-8°C

The enzyme from Sigma has been used in developing a protocol for ex vivo culture of mouse embryonic mammary buds. It has been used in the treatment of rat heart pieces during the isolation of mitochondria from rat heart. It has also been used for the isolation of dental pulp stem cells (DPSCs) by enzymatic hydrolysis. These cells have been compared with DPSCs isolated by explant method to analyse their stem cell and differentiation properties.
Dispase® II has been used for Fluorescence-Activated Cell Sorting (FACS). It has also been used for separating visceral yolk sac layers.