Merck PCR Buffer Set

PCR Buffer Set

suitable for PCR, pkg of 2 × 2 mL

Quality Level

100

포장

pkg of 2 × 2 mL

manufacturer/tradename

Roche

technique(s)

PCR: suitable

배송 상태

dry ice

저장 온도

−20°C

The kit-provided PCR buffer without MgCl2, in combination with the kit-provided MgCl2 stock solution, are used for the individual adjustment of the Mg2+ concentration. In most PCR applications, a concentration of 1.5 mM MgCl2 will yield satisfactory results with a dNTP concentration of 200 μM for each nucleotide. To optimize the PCR, titrate to determine the optimal Mg2+ concentration for high PCR specificity and amplicon yield.