Merck JumpStart REDAccuTaq LA DNA Polymerase

JumpStart™ REDAccuTaq® LA DNA Polymerase

Long and accurate hot-start Taq with inert dye, 10X buffer included

Quality Level

200

form

liquid

사용

sufficient for 250 reactions
sufficient for 50 reactions

특징

Long & Accurate PCR
dNTPs included: no
hotstart

농도

1 unit/μL

technique(s)

PCR: suitable

색상

red

input

purified DNA

application(s)

agriculture

배송 상태

wet ice

저장 온도

−20°C

JumpStart™ REDAccuTaq® LA DNA Polymerase contains AccuTaq long and accurate (LA) DNA polymerase, an inert red dye, and JumpStart Taq antibody. This enzyme is suitable for long-distance and high-fidelity PCR, multiplex PCR, and PCR amplification of targets with variable lengths, such as amplification of cDNA libraries. It enables the amplification from 0.25 to 22 kb for complex genomic DNA and up to 40 kb for less complex templates. JumpStart Taq antibody is designed to minimize non-specific amplification while increasing target yield. Unlike other Hot-start methods (i.e. chemical inactivation), the JumpStart Taq antibody does not require a pre-incubation step before cycling because polymerase activity is fully restored during the first denaturation cycle of the PCR reaction. The PCR product can be easily separated from the red dye by standard purification methods. The inert red dye has does not affect automated sequencing, restriction enzyme digestion, ligation, or other downstream applications. Its high fidelity makes it the enzyme of choice when performing amplifications where a low error frequency is critical, such as in RT-PCR and cloning.