Merck Titan One Tube RT-PCR Kit

Titan One Tube RT-PCR Kit

sufficient for 50 reactions, suitable for RT-PCR

1 step RT-PCR

Quality Level

100

사용

sufficient for 50 reactions

특징

dNTPs included: no
hotstart: no

manufacturer/tradename

Roche

파라미터

42-60 °C optimum reaction temp.

technique(s)

RT-PCR: suitable

input

purified RNA

저장 온도

−20°C

Titan™ One Tube RT-PCR Kit uses Avian Myeloblastosis Virus (AMV) reverse transcriptase for first strand cDNA synthesis and the Expand™ High Fidelity enzyme blend consisting of Taq DNA polymerase and Tgo DNA polymerase for amplification of cDNA by PCR. Tgo DNA polymerase possesses proofreading activity leading to an increased PCR fidelity. This kit reduces the error rate, allows the amplification of fragments up to 6 kb by RT-PCR, delivers increased sensitivity. It is considered faster than conventional two-step RT-PCR.