Merck JumpStart AccuTaq LA DNA Polymerase

JumpStart™ AccuTaq™ LA DNA Polymerase

Hot-start high fidelity Taq enzyme, 10X buffer included

JumpStart AccuTaq LA DNA Polymerase

Quality Level

200

form

liquid

사용

sufficient for 125 reactions
sufficient for 500 reactions

특징

Long & Accurate PCR
dNTPs included: no
hotstart

농도

2.5 unit/μL

technique(s)

PCR: suitable

색상

colorless

input

purified DNA

배송 상태

wet ice

저장 온도

−20°C

JumpStart™ AccuTaq™ LA DNA Polymerase is a combination of Sigma′s high-fidelity blend, AccuTaq long and accurate (LA) DNA Polymerase, and the Taq neutralizing JumpStart Taq antibody. This specially formulated enzyme is the best choice for long-distance and high-fidelity PCR, multiplex PCR, and PCR amplification of targets with variable lengths, such as amplification of cDNA libraries. JumpStart Taq antibody reversibly binds to the AccuTaq LA DNA Polymerase, inactivating it at room temperature. The increased temperature of the first denaturation cycle causes the complex to dissociate, restoring the enzyme activity. This hot-start mechanism provides increased specificity and higher target yield in comparison to standard amplification.