Merck GenElute-E Organic Solvent DNA Cleanup Kit

GenElute™-E Organic Solvent DNA Cleanup Kit

정제법

(Single-spin negative chromotography)
(Time: 3 minutes or less)

특징

Compatible Application (Suitable for most common downstream applications, including genotyping, PCR, and NGS)
Intended use (For the removal of organic solvent traces from DNA solutions)
Typical/expected yield (Varies by sample. Please reference user guide for more information.)

환경친화적 대안 제품 특성

Waste Prevention
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

환경친화적 대안 카테고리

Aligned

저장 온도

room temp

DNA/RNA preparation by traditional methods frequently contains residual phenol and/or chloroform. The GenElute™-E Organic Solvent DNA Cleanup Kit is a one-step cleanup kit for the depletion of organic solvent traces, including phenol, TRIzol reagent, chloroform, and ethanol, from DNA solutions. Ideally suited for a reliable clean-up when a universal preparation for NGS library prep is desired or required.

GenElute™-E kits are based on the principle of negative chromatography, where impurities like salt, detergent, proteins and organic solvents are removed by the column material and the purified nucleic acid is collected in the flow-through in just one single centrifugation step. This convenient procedure enables a significantly faster purification of nucleic acid samples than standard bind-wash-elute procedures, with less handling, drastically reduced plastic consumable usage, and no use of hazardous materials like chaotropic salts or organic solvents.

With conventional methods, traces of chaotropic salt and ethanol are usually coeluted with these purification procedures, which often leads to inhibition of real-time PCR experiments. GenElute™-E kits do not contain any chaotropic salts, organic solvents or EDTA, resulting in improved performance in downstream applications such as PCR.

Because of the negative chromatography principle, there is no limitation to the amount of DNA that is cleaned up. Maximum sample volume is 100 μl. The purified nucleic acid is eluted in water and can immediately be used for downstream applications. A 1x TE Buffer, pH 8.5, is supplied to enable storage of samples.