Merck DIG-High Prime DNA Labeling and Detection Starter Kit I

DIG-High Prime DNA Labeling and Detection Starter Kit I

sufficient for 12 labeling reactions, sufficient for 24 blots, suitable for hybridization, suitable for Northern blotting, suitable for Southern blotting

사용

sufficient for 12 labeling reactions
sufficient for 24 blots

Quality Level

100

포장

kit of 1 (7 components)

manufacturer/tradename

Roche

환경친화적 대안 제품 특성

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

technique(s)

Northern blotting: suitable
Southern blotting: suitable
hybridization: suitable

환경친화적 대안 카테고리

Aligned

배송 상태

dry ice

저장 온도

−20°C

The DIG High Prime DNA Labeling and Detection Starter Kit I uses digoxigenin (DIG), a steroid hapten, to label DNA probes for hybridization and subsequent color detection by enzyme immunoassay. This kit contains a ready-made blocking solution, combined stock solution of of nitroblue tetrazolium (NBT)/ 5-bromo-4-chloro-3-indolyl-phosphate (BCIP) and DIG Easy Hyb granules. The DIG-High Prime mixture includes stabilized Klenow enzyme, nucleotides, primers and reaction buffer, all in one convenient reagent. The sample material can be: DNA fragments of at least 100bp, linearized plasmid, cosmid or λDNA, or supercoiled DNA. The random primed DNA labeling method originally developed by Feinberg and Vogelstein is based on the hybridization of oligonucleotides of all possible sequences to the denatured DNA to be labeled. The input DNA serves as a template for the synthesis of labeled DNA and is not degraded during the reaction, making it possible to label minimal amounts of DNA (10ng) with this method. In this method, the complementary DNA strand is synthesized by Klenow polymerase using the 3′-OH termini of the random oligonucleotides as primers. Modified deoxyribonucleoside triphosphates, labeled with digoxigenin, present in the reaction are incorporated into the newly synthesized complementary DNA strand. This is a convenient kit for random-primed labeling of DNA templates with DIG-11-dUTP, alkali-labile, and color detection of the DIG-labeled hybrids. We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.