Merck DIG RNA Labeling Mix

DIG RNA Labeling Mix

sufficient for 20 reactions, solution

form

solution

Quality Level

100

사용

sufficient for 20 reactions

포장

pkg of 40 μL

manufacturer/tradename

Roche

환경친화적 대안 제품 특성

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

환경친화적 대안 카테고리

Aligned

배송 상태

dry ice

저장 온도

−20°C

Labeling efficiency: Approximately 10μg of full-length digoxigenin-labeled RNA is transcribed from 1μg linear template DNA.
Assay Time: 135 minutes
Sample Materials
Linearized plasmid DNA:
The DNA to be transcribed is cloned into the polylinker site of an appropriate transcription vector which contains adjacent to the polylinker a promoter for SP6, T7 or T3 RNA polymerase. For the synthesis of ‘run off′ transcripts the plasmid is linearized by a restriction enzyme. Restriction enzymes creating 5′-overhangs should be used; 3′ overhangs should be avoided. The linearized template DNA should be purified by phenol chloroform extraction and ethanol precipitation, to avoid RNase contamination. For ′run around′ transcription circular plasmid DNA is used.
PCR product:
PCR-fragments which contain RNA polymerase promoter sequences can also be used as templates for transcription. Purification of the PCR fragment by HighPure column purification prior to transcription is recommended.