Merck Microbial DNA standard from Deinococcus radiodurans

Microbial DNA standard from Deinococcus radiodurans

Suitable for PCR, sequencing and NGS, 10 ng/μL

Quality Level

200

form

liquid

농도

10 ng/μL

technique(s)

PCR: suitable

배송 상태

ambient

저장 온도

−20°C

Standardization of sample analysis is currently needed in microbiome genomics research workflow. Lack of standardization can lead to biases and errors in common processes during sample preparation and analysis such as sample amplification, sequencing and bioinformatics analyses.1 Deinococcus rediodurans genomic DNA microbial standard can serve as standard for benchmarking the performance along the workflow of microbiomics or meta-genomics analyses and as a tool to increase reproducibility and allow comparison of results obtained by different labs.Since Deinococcus radiodurans is not a typical resident of the human microbiota it can serve as a spike-in standard in human microbiota genomics workflow analysis.
D_einococcus radiodurans_ is an extremophilic, heterotrophic, non-motile, non-spore-forming, aerobic tetracoccus bacterium. 2 The cell envelope of D_einococcus radiodurans_ stains as a gram-positive, but the two membranes that separated by a peptidoglycan layer, makes it more similar to typical gram-negative bacteria. 3 Deinococcus radiodurans is an extremophilic microorganism that can survive harsh conditions such as cold, high temperature, acid, alkali, resistance to desiccation, oxidative stress, ionizing radiation and ultraviolet radiation. 3 Mechanisms responsible for these extremophilic characteristics include: efficient repair of DNA damage 4, 5, Nucleotides Excision Repair (NER) 6, unique DNA mismatch repair (MMR) method 7 and efficient cellular mechanisms such as proteolytic protein for removal damaged and misfolded proteins, Nudix hydrolase 8 a broad-spectrum of transcription factor and a unique regulatory protein, which stimulates recA gene transcription after exposure to ionizing radiation 9.The large tolerance of the extremophile, D_einococcus radiodurans_ to the extraordinary conditions makes this bacterium as a tool for metabolic engineering 10.

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