Merck Water,microbial DNA free

Water,microbial DNA free

Suitable for PCR, sequencing and NGS,DNA free

UV-treated water, Microbiome water, DNA-free water, Filter-sterilized water, Microbial DNA-free water

Quality Level

200

form

liquid

특징

DNA free

technique(s)

PCR: suitable

배송 상태

ambient

저장 온도

room temp

Microbial DNA-free water is filter-sterilized and UV- treated. It undergoes stringent quality control testing to ensure that it is free of any detectable levels of DNA. The quality tests guarantee the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.

Provided in 10 vials of 1.5 ml, enabling use of 1 tube per PCR setup to avoid contamination of your master stock.

Progression in Next Generation Sequencing (NGS) technology now allows identification and sequencing of microbes in relatively low quantities and in complex samples. Therefore, NGS is the method of choice for population identification in microbiome samples, as most of them include large variety of microbes and in some cases also host cells. Still, biases and errors are common in this workflow and can be introduced in different steps along this process. Reagents used must be free of any microbial contaminant to avoid introduction of exogenous error to the samples especially these with relatively low microbial counts