Merck Cas9 RFP Lentiviral Particles

Cas9 RFP Lentiviral Particles

form

liquid

포장

pkg of 8 x 25 μL (aliquots)

농도

≥5 x 10 8 VP/ml virus (via p24 assay)

application(s)

CRISPR

리포터 유전자

RFP

배송 상태

dry ice

저장 온도

−20°C

Ready-to-use Cas9-RFP lentiviral particles enable immediate transduction of a wide range of cell lines. Cas9-RFP lentiviral particles efficiently and stably integrate Cas9 and RFP linked by a 2A peptide and driven by the EF1 alpha promoter (EF1a-Cas9-2A-GFP) for strong expression in both dividing and non-dividing cell lines. They are ideal for avoiding the tedious lentivirus production process and are one part of a two part CRISPR system with individual Cas9 and gRNA expression vectors. For easy fluorescence microscopy a MOI greater than 200 should be employed.

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