Merck MISSION Lenti microRNA Inhibitor, Mouse

MISSION® Lenti microRNA Inhibitor, Mouse

mmu-miR-3092-3p

Tough Decoy (TuD) Design

품질 등급

200

제품 라인

MISSION®

형태

Liquid

농도

≥1×10⁶ VP/ml (via p24 assay)

성숙 서열

GAAUGGGGCUGUUUCCCCUCC

Sanger 성숙/소수 수납 번호

MIMAT0014906

microRNA 수납 번호

MI0014085

배송 상태

Dry ice

저장 온도

−70°C

제품 설명

Individual lenti microRNA inhibitors are designed using a proprietary algorithm, based on the work of Haraguchi, T. et al., in collaboration with Dr. Hideo Iba (University of Tokyo).
This algorithm utilizes the Tough Decoy (TuD) design.
miRNA regulate gene expression through mechanisms such as translational repression, mRNA cleavage, and deadenylation.

The lentiviral microRNA inhibitors are cloned into the TRC2-pLKO-puro vector.
Co-transfection with compatible packaging plasmids produces viral particles suitable for transduction of mammalian cells.
The vector includes the Woodchuck Hepatitis Post-Transcriptional Regulatory Element (WPRE) for enhanced transgene expression and a puromycin resistance gene for selection.

주요 특징

• 강력한 miRNA 억제 효과 제공
• 렌티바이러스 전달 포맷으로 다양한 세포주에 효율적 전달
• 반복 형질감염 없이 장기적 억제 가능