Merck MISSION Lenti microRNA Inhibitor, Mouse

MISSION® Lenti microRNA Inhibitor, Mouse

mmu-miR-20b-3p

Tough Decoy (TuD)

제품 개요

Individual lenti microRNA inhibitors are designed using a proprietary algorithm based on the work of Haraguchi, T. et al., in collaboration with Dr. Hideo Iba (University of Tokyo).
This algorithm utilizes the Tough Decoy (TuD) design to effectively inhibit target miRNA.
miRNAs regulate gene expression through translational repression, mRNA cleavage, and deadenylation.

The lentiviral microRNA inhibitors are cloned into the TRC2-pLKO-puro vector.
When co-transfected with compatible packaging plasmids, viral particles are produced for mammalian cell transduction.
The vector includes the Woodchuck Hepatitis Post-Transcriptional Regulatory Element (WPRE) for enhanced transgene expression and a puromycin resistance gene for selection.

주요 특징

• 목표 miRNA의 강력하고 특이적인 억제
• Lentiviral 전달로 다양한 세포 유형에 효율적 적용 가능
• 반복적인 형질감염 없이 장기 억제 가능
• Tough Decoy(TuD) 기반 설계로 높은 안정성 확보

제품 사양

참고 문헌

• Haraguchi, T. et al., University of Tokyo – Development of the Tough Decoy (TuD) design for miRNA inhibition.

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