Merck MISSION Lenti microRNA Inhibitor, Mouse

MISSION® Lenti microRNA Inhibitor, Mouse

타깃 microRNA

• mmu-miR-302c-5p

디자인

• Tough Decoy (TuD) 기반 설계

품질 등급

• 200

제품 라인

• MISSION®

물리적 형태

• Liquid

농도

• ≥1×10⁶ VP/ml (via p24 assay)

성숙 서열

• GCUUUAACAUGGGGUUACCUGC

Sanger 성숙/소수 수납 번호

• MIMAT0003375

microRNA 수납 번호

• MI0003717

배송 상태

• Dry ice

저장 온도

• −70°C

제품 설명

Individual lenti microRNA inhibitors are designed using a proprietary algorithm based on the work of Haraguchi, T. et al., in collaboration with Dr. Hideo Iba (University of Tokyo).
This algorithm utilizes the Tough Decoy (TuD) design for potent inhibition of target miRNA.

miRNA are known to regulate gene expression through translational repression, mRNA cleavage, and deadenylation.
The lentiviral microRNA inhibitors are cloned into the TRC2-pLKO-puro vector.
Co-transfection with compatible packaging plasmids produces viral particles suitable for transduction of mammalian cells.

Additionally, the Woodchuck Hepatitis Post-Transcriptional Regulatory Element 2 (WPRE) is included for enhanced transgene expression.
The vector also carries a puromycin resistance gene for cell selection.

주요 특징

• 강력한 miRNA 억제 효과 제공
• 렌티바이러스 전달 포맷으로 다양한 세포형에 효율적 적용 가능
• 반복적인 트랜스펙션 없이 장기적 억제 가능
• 퓨로마이신 저항성 유전자를 통한 선택적 세포 선별 가능