Merck MISSION Lenti microRNA Inhibitor, Mouse

MISSION® Lenti microRNA Inhibitor, Mouse

대상 microRNA

• mmu-miR-3096a-5p

디자인

• Tough Decoy (TuD)

품질 등급

• 200
  M-Clarity Program 안내 보기

제품 라인

• MISSION®

제형 (Form)

• Liquid

농도

• ≥1×10⁶ VP/ml (via p24 assay)

성숙 서열 (Mature Sequence)

• UGGCCAAGGAUGAGAACU

Sanger 성숙/소수 수납 번호

• MIMAT0014913

저장 온도

• −70 °C

제품 설명

Individual lenti microRNA inhibitors are designed using a proprietary algorithm based on the work of Haraguchi, T. et al., in collaboration with Dr. Hideo Iba (University of Tokyo). This algorithm utilizes the Tough Decoy (TuD) design.

miRNAs regulate gene expression through mechanisms such as translational repression, mRNA cleavage, and deadenylation. The lentiviral microRNA inhibitors are cloned into the TRC2-pLKO-puro vector. Co-transfection with compatible packaging plasmids enables production of viral particles for efficient transduction of mammalian cells.

The vector includes the Woodchuck Hepatitis Post-Transcriptional Regulatory Element (WPRE) for enhanced transgene expression and carries a puromycin resistance gene for selection.

주요 특징

• 원하는 miRNA의 강력한 억제 가능
• 다양한 세포 유형에 효율적인 렌티바이러스 전달
• 반복 형질감염 없이 장기 억제 유지
• puromycin 내성 유전자를 통한 세포 선택 용이