Merck MISSION Lenti microRNA Inhibitor, Mouse

MISSION® Lenti microRNA Inhibitor, Mouse

mmu-miR-299a-5p

Tough Decoy (TuD) 기반 설계

제품 개요

Individual lenti microRNA inhibitors are designed using a proprietary algorithm based on the work of Haraguchi, T. et al., in collaboration with Dr. Hideo Iba (University of Tokyo).
This algorithm utilizes the Tough Decoy (TuD) design to achieve potent inhibition of target miRNA.
miRNA regulate gene expression via translational repression, mRNA cleavage, and deadenylation.

The lentiviral microRNA inhibitors are cloned into the TRC2-pLKO-puro vector.
Co-transfection with compatible packaging plasmids produces viral particles for mammalian cell transduction.
The vector includes the Woodchuck Hepatitis Post-Transcriptional Regulatory Element 2 (WPRE) for enhanced expression and carries a puromycin resistance gene for selection.

주요 특징

• 강력한 miRNA 억제 효과
• 다양한 세포 유형에 효율적 렌티바이러스 전달
• 반복 형질감염 없이 장기적 억제 유지
• puromycin 내성 유전자를 통해 안정적 세포 선택 가능

제품 사양

제품 이미지

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