Merck PSF-MINCMV-FLUC - MINIMAL CMV PROMOTER LUCIFERASE PLASMID

PSF-MINCMV-FLUC - MINIMAL CMV PROMOTER LUCIFERASE PLASMID

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

form

buffered aqueous solution

분자량

size 5383 bp

박테리아 선정

kanamycin

복제개시점

pUC (500 copies)

펩타이드 절단

no cleavage

프로모터

Promoter name: CMV
Promoter activity: minimal promoter
Promoter type: mammalian

리포터 유전자

firefly luciferase

배송 상태

ambient

저장 온도

−20°C

The expression vector contains the minimal CMV promoter driving expression of Firefly luciferase. The vector normally mediates low levels of expression in most mammalian cells however there is an MCS immediately upstream of the minimal promoter that allows additional regulatory components to be inserted. These can include cell- or tissue-selective enhancers or repressors such as transcription factor binding sites or can be made responsive to external stimuli such as drugs. In this way a range of tuneable reporter systems can be prepared.

Promoter Expression Level: This cloning vector contains the minimal CMV immediate early promoter with a multiple cloning site immediately upstream that allows transcription factor binding sites to be inserted. This allows tissue specific or physiologically responsive promoters to be created.