Merck PSF-MINCMV-PURO - MINIMAL CMV PUROMYCIN VECTOR

PSF-MINCMV-PURO - MINIMAL CMV PUROMYCIN VECTOR

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

form

buffered aqueous solution

분자량

size 4330 bp

박테리아 선정

kanamycin

포유류 세포 선정

puromycin

복제개시점

pUC (500 copies)

펩타이드 절단

no cleavage

프로모터

Promoter name: CMV
Promoter activity: minimal promoter
Promoter type: mammalian

리포터 유전자

none

배송 상태

ambient

저장 온도

−20°C

In this plasmid the minimal CMV promoter regulates expression of the puromycin resistance gene providing basal levels of expression in most mammalian cells. An upstream MCS enables additional regulatory elements to be introduced upstream of the minimal promoter to make it responsive to cell-associated transcription factors (either stimulatory or repressive) or externally-applied drugs. In this way a range of sophisticated vectors can be prepared that provide puromycin resistance under carefully-defined conditions.

Promoter Expression Level: This plasmid contains the minimal CMV immediate early promoter with a multiple cloning site immediately upstream that allows transcription factor binding sites to be inserted. This allows tissue specific or physiologically responsive promoters to be created.