Merck PSF-OXB12 - INTERMEDIATE STRENGTH BACTERIAL PLASMID

PSF-OXB12 - INTERMEDIATE STRENGTH BACTERIAL PLASMID

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

재조합

expressed in E. coli

form

buffered aqueous solution

분자량

size 3857 bp

박테리아 선정

kanamycin

복제개시점

pUC (500 copies)

펩타이드 절단

no cleavage

프로모터

Promoter name: OXB12
Promoter activity: constitutive
Promoter type: bacterial

리포터 유전자

none

배송 상태

ambient

저장 온도

−20°C

This plasmid contains a promoter called OXB12 which is designed for constitutive protein production in E. coli. It was created by random mutagenesis of the E. coli RecA promoter with the LexA repressor site removed. The promoter was found to demonstrate intermediate levels of expression. This plasmid is part of our constitutive E. coli promoter range that extends from OXB1 (low expression) to OXB20 (high expression).

Promoter Expression Level: This plasmid contains a medium strength constitutive E. coli promoter that was derived from the RecA promoter by random mutagenesis. It is part of our constitutive bacterial promoter range. This promoter (OXB12) shows the intermediate levels of expression in the range with OXB1 showing the lowest level and OXB20 showing the highest level of expression. They require no inducing agent for expression.