Merck PSF-TEFI-TPI1-ILUMENA - SECRETED LUCIFERASE YEAST VECTOR
다른 상품 둘러보기
PSF-TEFI-TPI1-ILUMENA - SECRETED LUCIFERASE YEAST VECTOR
plasmid vector for molecular cloning
expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid
form
buffered aqueous solution
분자량
size 8209 bp
박테리아 선정
kanamycin
복제개시점
2Micron
pUC (500 copies)
펩타이드 절단
no cleavage
프로모터
Promoter name: TEF1
Promoter activity: constitutive
Promoter type: yeast
리포터 유전자
iLumena luciferase
배송 상태
ambient
저장 온도
−20°C
효모 선정
uracil
PSF-TEFI-TPI1-ILUMENA - SECRETED LUCIFERASE YEAST VECTOR plasmid encodes a secreted luciferase reporter gene (iLumena) that allows for the measurement of plasmid activity in the supernatant of cell cultures from Saccharomyces cerevisiae yeast. This reporter is unique to our product range. The reporter uses coelenterazine as the substrate and substrates desgiend for guassia luciferase will work well with this reporter. Reporter gene expression is driven from the TPI1 cassette. There is a TEF1 promoter upstream of the multiple cloning site to drive the expression of a gene of intererst. These promoters show similar levels of expression. The plasmid is maintained in yeast cells using the URA3 selection method whereby the yeast strain used is defective for this gene (which is essential for uracil synthesis) and the cells are grown in uracil deficient media.
Promoter Expression Level: This molecular cloning vector contains the yeast translation elongation factor 1 promoter. It is the strongest promoter that we provide for expression in Saccharomyces cerevisiae. It also contains the strong yeast constitutive triosephosphate isomerase (TPI1) gene promoter to drive the expression of the reporter gene. The TPI promoter demonstrates similar levels of expression to the translation elongation factor 1 promoter (TEF-1).
배송/결제/교환/반품 안내
배송 정보
기본 배송비 |
| 교환/반품 배송비 |
|
---|---|---|---|
착불 배송비 |
| ||
교환/반품 배송비 |
|
결제 및 환불 안내
결제 방법 |
|
---|---|
취소 |
|
반품 |
|
환급 |
|
교환 및 반품 접수
교환 및 반품 접수 기한 |
|
---|---|
교환 및 반품 접수가 가능한 경우 |
|
교환 및 반품 접수가 불가능한 경우 |
|
교환 및 반품 신청
교환 절차 |
|
---|---|
반품 절차 |
|