
Merck PSF-MINCMV-FRCFP - MINIMAL CMV CFP CLONING PLASMID
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PSF-MINCMV-FRCFP - MINIMAL CMV CFP CLONING PLASMID
plasmid vector for molecular cloning
expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid
form
buffered aqueous solution
분자량
size 4441 bp
박테리아 선정
kanamycin
복제개시점
pUC (500 copies)
펩타이드 절단
no cleavage
프로모터
Promoter name: CMV
Promoter activity: minimal promoter
Promoter type: mammalian
리포터 유전자
CFP
배송 상태
ambient
저장 온도
−20°C
The Frosty Cyan Fluorescence Protein (FrCFP) is under regulatory control of the minimal CMV promoter in this plasmid giving low level expression of the FrCFP transgene in most types of mammalian cells. Positive or negative regulatory elements can be introduced upstream of the minimal promtoer using a specially-positioned MCS to endow your required responsiveness. For example transcription factor binding sites could be introduced to provide tissue-selective transgene expression. Similarly drug-responsive sites could be introduced to provide expression regulated by externally-applied drugs.
Promoter Expression Level: This plasmid contains the minimal CMV immediate early promoter with a multiple cloning site immediately upstream that allows transcription factor binding sites to be inserted. This allows tissue specific or physiologically responsive promoters to be created.
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