Merck PSF-TEFI-TPI1-CFP-URA3 - YEAST CFP REPORTER VECTOR

PSF-TEFI-TPI1-CFP-URA3 - YEAST CFP REPORTER VECTOR

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

form

buffered aqueous solution

분자량

size 8284 bp

박테리아 선정

kanamycin

복제개시점

2Micron
pUC (500 copies)

펩타이드 절단

no cleavage

프로모터

Promoter name: TEF1
Promoter activity: constitutive
Promoter type: yeast

리포터 유전자

CFP

배송 상태

ambient

저장 온도

−20°C

효모 선정

uracil

A cyan fluorescent protein (CFP) reporter gene plasmid that allows for the expression of a transgene alongside the CFP gene. The CFP variant in this plasmid was developed by DNA2. 0 and is called Frosty CFP. Expression of the reporter gene is mediated by the TPI promoter whilst the expression of a gene of interest would be controlled by the TEF1 promoter. These are both endogenous yeast promoters that require no induction for activity. They demonstrate approximately equal expression levels. The plasmid also contains the URA3 selection cassette which encodes an essential component for uracil synthesis. This means the that plasmid can be maintained in media that is deficient in uracil using yeast strains that are defective for the URA3 cassette.

Promoter Expression Level: This plasmid contains the yeast translation elongation factor 1 promoter. It is the strongest promoter that we provide for expression in Saccharomyces cerevisiae. It also contains the strong yeast constitutive triosephosphate isomerase (TPI1) gene promoter to drive the expression of the reporter gene. The TPI promoter demonstrates similar levels of expression to the translation elongation factor 1 promoter (TEF-1).