Merck PSF-TEFI-TPI1-CFP-URA3 - YEAST CFP REPORTER VECTOR
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PSF-TEFI-TPI1-CFP-URA3 - YEAST CFP REPORTER VECTOR
plasmid vector for molecular cloning
expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid
form
buffered aqueous solution
분자량
size 8284 bp
박테리아 선정
kanamycin
복제개시점
2Micron
pUC (500 copies)
펩타이드 절단
no cleavage
프로모터
Promoter name: TEF1
Promoter activity: constitutive
Promoter type: yeast
리포터 유전자
CFP
배송 상태
ambient
저장 온도
−20°C
효모 선정
uracil
A cyan fluorescent protein (CFP) reporter gene plasmid that allows for the expression of a transgene alongside the CFP gene. The CFP variant in this plasmid was developed by DNA2. 0 and is called Frosty CFP. Expression of the reporter gene is mediated by the TPI promoter whilst the expression of a gene of interest would be controlled by the TEF1 promoter. These are both endogenous yeast promoters that require no induction for activity. They demonstrate approximately equal expression levels. The plasmid also contains the URA3 selection cassette which encodes an essential component for uracil synthesis. This means the that plasmid can be maintained in media that is deficient in uracil using yeast strains that are defective for the URA3 cassette.
Promoter Expression Level: This plasmid contains the yeast translation elongation factor 1 promoter. It is the strongest promoter that we provide for expression in Saccharomyces cerevisiae. It also contains the strong yeast constitutive triosephosphate isomerase (TPI1) gene promoter to drive the expression of the reporter gene. The TPI promoter demonstrates similar levels of expression to the translation elongation factor 1 promoter (TEF-1).
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