Merck PSF-MINCMV-KRYFP - MINIMAL CMV YFP EXPRESSION VECTOR

PSF-MINCMV-KRYFP - MINIMAL CMV YFP EXPRESSION VECTOR

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

form

buffered aqueous solution

분자량

size 4441 bp

박테리아 선정

kanamycin

복제개시점

pUC (500 copies)

펩타이드 절단

no cleavage

프로모터

Promoter name: CMV
Promoter activity: minimal promoter
Promoter type: mammalian

리포터 유전자

YFP

배송 상태

ambient

저장 온도

−20°C

In this plasmid Kringle Yellow Fluorescence Protein (YFP) is under transcriptional control of the minimal CMV promoter giving low level expression in many mammalian cells. An MCS upstream of the minimal promoter can be used to introduce regulatory elements such as transcription factor-binding sites to endow tissue-selective expression or responsiveness to exogenously-applied drugs. This permits a range of sophisticated reporter systems to be prepared using this vector.

Promoter Expression Level: This plasmid contains the minimal CMV immediate early promoter with a multiple cloning site immediately upstream that allows transcription factor binding sites to be inserted. This allows tissue specific or physiologically responsive promoters to be created.