Merck PSF-CMV-MULAMBDA - MOUSE LAMBDA LIGHT CHAIN ANTIBODY VECTOR
Mouse lambda light chain antibody expression vector for molecular cloning. Contains CMV promoter for strong mammalian expression. Kanamycin selection marker with pUC origin. Supplied in buffered aqueous solution, stored at −20°C.
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PSF-CMV-MULAMBDA - Mouse Lambda Light Chain Antibody Vector
개요
This plasmid vector is designed for molecular cloning and antibody expression. It contains the mouse (Mus musculus) lambda antibody light chain constant region, enabling the creation of full-length light chain antibody expression constructs. The constant region is positioned downstream of a BseRI restriction site, allowing seamless insertion of variable regions by adding a complementary BseRI site.
주요 특징
- Expression vector for mammalian systems
- Contains mouse lambda light chain constant region
- CMV promoter for strong constitutive expression
- Kanamycin resistance for bacterial selection
- pUC origin (high copy number)
스펙 정보
| 항목 | 내용 |
|---|---|
| 형태(Form) | Buffered aqueous solution |
| 크기(Size) | 4573 bp |
| 박테리아 선택 마커 | Kanamycin |
| 복제 개시점 | pUC (500 copies) |
| 펩타이드 절단 | No cleavage |
| 프로모터 이름 | CMV |
| 프로모터 활성 | Constitutive |
| 프로모터 타입 | Mammalian |
| 리포터 유전자 | None |
| 배송 상태 | Ambient |
| 저장 온도 | −20°C |
추가 설명
Promoter Expression Level:
This plasmid contains the mammalian CMV promoter to drive gene expression. Tests across various cell types show CMV as one of the strongest promoters. However, long-term cultures may experience silencing due to methylation.
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