Merck PSF-TEFI-TPI1-BLAST - BLASTICIDIN RESISTANT YEAST VECTOR

PSF-TEFI-TPI1-BLAST - BLASTICIDIN RESISTANT YEAST VECTOR

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

form

buffered aqueous solution

분자량

size 6880 bp

박테리아 선정

kanamycin

복제개시점

2Micron
pUC (500 copies)

펩타이드 절단

no cleavage

프로모터

Promoter name: TEF1
Promoter activity: constitutive
Promoter type: yeast

리포터 유전자

none

배송 상태

ambient

저장 온도

−20°C

PSF-TEFI-TPI1-BLAST - Blasticidin resistant yeast vector is a blasticidin resistant Saccharomyces cerevisiae expression plasmid. This expression vector contains the yeast EF1-Alpha promoter to drive transgene expression. Saccharomyces cerevisiae yeast expression plasmid using the strong constitutive TEF1 promoter to drive protein production from a gene of interest. The plasmid also contains an antibiotic resistance cassette that provides resistance to blasticidin. This cassette is driven from the strong constitutive TPI yeast promoter.

Promoter Expression Level: This plasmid contains the yeast translation elongation factor 1 promoter. It is the strongest promoter that we provide for expression in Saccharomyces cerevisiae. It also contains the strong yeast constitutive triosephosphate isomerase (TPI1) gene promoter to drive the antibiotic resistance gene. The TPI promoter demonstrates similar levels of expression to the translation elongation factor 1 promoter (TEF-1).