Merck PSF-SP6 - SP6 PROMOTER PLASMID

PSF-SP6 - SP6 PROMOTER PLASMID

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

form

buffered aqueous solution

분자량

size 3862 bp

박테리아 선정

kanamycin

복제개시점

pUC (500 copies)

펩타이드 절단

no cleavage

리포터 유전자

none

배송 상태

ambient

저장 온도

−20°C

This vector allows transcription of un-polyadenylated and un-capped RNA using the SP6 bacteriophage polymerase. Some in vitro transcription kits will enable the production poly-adenylated and capped RNA using this vector; however this will depend on the kit used. SnapFast vectors do not contain an SP6 terminator and so to prevent the polymerase from circling the plasmid it must be cleaved at a restriction site that is 3 prime to the end of your gene to allow run-off of the polymerase. If you are expressing your gene in a vaccinia virus-free mammalian SP6 system it will require an IRES upstream of your gene because of the absence of a 5 prime cap on the RNA produced.

Promoter Expression Level: