Merck PSF-LENTI - LENTIVIRUS PUROMYCIN CMV PLASMID

PSF-LENTI - LENTIVIRUS PUROMYCIN CMV PLASMID

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

form

buffered aqueous solution

분자량

size 33440 bp

박테리아 선정

ampicillin

복제개시점

pUC (500 copies)

펩타이드 절단

no cleavage

리포터 유전자

none

배송 상태

ambient

저장 온도

−20°C

PSF-LENTI - lentivirus puromycin CMV plasmid contains a HIV1 based lentivirus expression vector that can be used for the stable inteagration of genes into both dividing and non-dividing cells. The plasmid is based on the commonly used pCCL lentivirus backbone and contains a range of modifications to make cloning easier. The plasmid vector contains an expanded multiple cloning site and is compatible with most of the plasmids we provide in our product range which allows sections to compiled to create complex expression vectors. This plasmid contains the CMV promoter to drive the expression of a gene of interest followed by a multiple cloning site and the PGK promoter to drive puromycin resistance.

Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture. The murine phosphoglycerate kinase (PGK) promoter driving puromycin expression allows for long term stable expression in vitro and in vivo in most types.