Merck Dey-Engley neutralizing broth

Dey-Engley neutralizing broth

suitable for microbiology, NutriSelect® Plus

D/E Neutralizing Broth

Quality Level

200

무균

non-sterile

form

powder

유통기한

limited shelf life, expiry date on the label

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

최종 pH

7.6±0.2 (25 °C)

application(s)

environmental
food and beverages
pharmaceutical
sterility testing
surface monitoring
microbiology

저장 온도

2-8°C

적합성

nonselective for Bacillus spp.
nonselective for Escherichia coli
nonselective for Pseudomonas spp.
nonselective for Salmonella spp.
nonselective for Staphylococcus spp.
nonselective for bacteria (General Media)
nonselective for coliforms

Dey-Engley Neutralizing Broth is used in disinfectant testing where the neutralization of the antiseptics and disinfectants is important for determining its bactericidal activity. The broth consists of tryptone that provides nitrogen and carbon sources, long chain amino acids, vitamins, and other essential nutrients. Dextrose is an energy source. Yeast extract is also a rich source of vitamin B-complex. Sodium bisulfite neutralizes aldehydes; sodium thioglycolate neutralizes mercurial; sodium thiosulfate neutralizes iodine and chlorine; lecithin neutralizes quaternary ammonium compounds; and polysorbate 80, a non-ionic surface-active agent, neutralizes substituted phenolics. Bromocresol purple is an indicator for dextrose utilization. Those organisms that ferment dextrose will turn the medium from purple to yellow. Growth of Pseudomonas sp., which does not ferment dextrose, can be detected by the formation of a pellicle on the surface of the broth.