Merck Fibronectin Fragment III1-C human

Fibronectin Fragment III1-C human

제품 개요

Recombinant fibronectin fragment III1-C, expressed in E. coli, lyophilized powder.
Product code: FF III1-C

제품 설명

Fibronectins are composed of two subunits linked by disulfide bonds at the C-terminal. In the extracellular matrix, fibronectins form high molecular weight polymers through additional disulfide bonding. Each subunit consists of repeating modules of three types: I, II, and III.

• Type I: 12 repeats (~45 amino acids), contains two disulfide bonds
• Type II: 2 repeats (~60 amino acids), contains two disulfide bonds
• Type III: 15–17 repeats (~90 amino acids), lacks disulfide bonds but has two free sulfhydryl groups per subunit

A newly identified region, the type III1 repeat cloned from human placenta cDNA, is involved in matrix formation. Experiments using antibodies against fibronectin domains revealed that the type III1 and type I modules are critical for fibronectin assembly. A 14 kDa fragment from the first two type III repeats was shown to inhibit fibronectin matrix assembly.

Recombinant fragment III1-C, modeled after the C-terminal two-thirds of the III1 repeat, binds to fibronectin and induces spontaneous disulfide crosslinking of fibronectin molecules into multimers resembling matrix fibrils.