Thermo Fisher Scientific POLR2E Monoclonal Antibody (OTI3C5), TrueMAB
POLR2E 단백질을 인식하는 Mouse Monoclonal 항체로, Western blot과 Immunocytochemistry에 적합합니다. HEK293T 세포에서 발현된 human POLR2E 전체 길이 단백질을 면역원으로 제작되었습니다. 동결건조 형태로 제공되며, 재구성 후 약 1 mg/mL 농도로 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3C5 |
| Immunogen | Full length human recombinant protein of human POLR2E produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
The POLR2E gene encodes the fifth largest subunit of RNA polymerase II, responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by other DNA-directed RNA polymerases and exists in two-fold molar excess over other subunits. It interacts with a hepatitis virus transactivating protein, suggesting that transcriptional activators may engage the polymerase through this subunit. A pseudogene is located on chromosome 11.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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