Thermo Fisher Scientific RASSF1 Monoclonal Antibody (OTI8E4), TrueMAB
RASSF1 단백질을 인식하는 Mouse monoclonal antibody (Clone OTI8E4). Western blot, IHC, ICC/IF, Flow cytometry에 적합. Human 시료 반응성. Lyophilized 형태로 제공되며, 재구성 후 1 mg/mL 농도. 종양 억제 관련 연구에 활용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI8E4 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1–341 of human RASSF1 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
The RASSF1 gene encodes a protein similar to RAS effector proteins. Loss or altered expression of this gene is associated with various cancers, suggesting its tumor suppressor function. Inactivation of this gene correlates with hypermethylation of its CpG-island promoter region. The encoded protein interacts with DNA repair protein XPA and inhibits cyclin D1 accumulation, inducing cell cycle arrest. Seven alternatively spliced transcript variants encoding distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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