Thermo Fisher Scientific IKK beta Polyclonal Antibody

Thermo Fisher Scientific IKK beta Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.

Anti-IKKβ may react non-specifically with other proteins. Control peptide (code #100-401-220p) will compete only with the specific reaction of antiserum with the IKKβ subunit.

Target Information

Phosphorylation of serine residues on the I-kappa-B proteins by IKK kinases, including IKBKB, marks them for destruction via the ubiquitination pathway, allowing activation of the NF-kappa-B complex.
IKK beta (Inhibitor of nuclear factor kappa-B kinase subunit beta) is a serine kinase that plays an essential role in the NF-κB signaling pathway, activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damage, or other cellular stresses.
IKK beta acts as part of the canonical IKK complex in the conventional pathway of NF-κB activation and phosphorylates inhibitors of NF-κB on two critical serine residues. These modifications lead to polyubiquitination and degradation of inhibitors by the proteasome. Consequently, free NF-κB translocates into the nucleus and activates transcription of genes involved in immune response, growth control, and protection against apoptosis.
Mutations affecting the IKK beta gene can result in Immunodeficiency 15 (IMD15).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.