Thermo Fisher Scientific Phospho-STAT3 (Ser727) Polyclonal Antibody
STAT3 단백질의 Ser727 인산화 부위를 특이적으로 인식하는 rabbit polyclonal 항체. WB, IHC, ICC, Flow, ELISA 등 다양한 응용에 사용 가능. Protein A로 정제된 1 mg/mL 액상 형태로 제공되며, -20°C에서 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:20–1:100 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rabbit, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic phosphopeptide derived from human STAT3 around Ser727 phosphorylation site |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
STAT3 (Signal Transducer and Activator of Transcription 3) belongs to the STAT protein family, which are phosphorylated by receptor-associated kinases and translocate to the nucleus to act as transcription factors in response to cytokines and growth factors. Coactivators such as CREB-binding protein are required for STAT3 transcriptional activation. STAT3 can be activated by interferons (α, γ), EGF, PDGF, and IL-6. Phosphorylation at tyrosine 705 by JAK1 and JAK2 is critical for dimer formation, nuclear translocation, and DNA binding activity. The STAT3 gene is located on chromosome 17q in humans. It regulates genes involved in cell growth and apoptosis, and knockout of STAT3 in mice is embryonically lethal. STAT3 activity is modulated by Rac1 and inhibited specifically by PIAS3. Three alternatively spliced isoforms of STAT3 have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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