Thermo Fisher Scientific Phospho-Stat4 (Tyr693) Polyclonal Antibody
Phospho-Stat4 (Tyr693) 폴리클로날 항체로 인간, 마우스, 랫트에서 반응. Western blot, IHC, ICC/IF, ELISA 등 다양한 응용 가능. KLH 결합 합성 인산화 펩타이드로 면역원 제작. Protein A 정제, -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:5,000–1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic phosphopeptide derived from human Stat4 around the phosphorylation site of Tyr693 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
STAT4 was originally identified using degenerate primers complementary to sequences encoding conserved regions of other STAT proteins. The STAT4 protein is most similar to STAT1 (52%) and STAT3 (47%). Functionally, STAT4 can be tyrosine phosphorylated by Jak1 or Jak2, forming homo- or heterodimers with related STAT family members. Tyrosine-phosphorylated STAT4 binds the IFN-gamma activated site (GAS), and serine phosphorylation is required for maximal transcriptional activity.
STAT4 expression is restricted to the thymus, spleen, and testis. STAT4 is activated by interleukin 12 (IL-12), which is essential for IFN-gamma induction and development of Th1 responses for effective defense against intracellular pathogens. STAT4-deficient mice show impaired IL-12-dependent Th1 development and enhanced Th2 cell formation. In response to viral infection, IFN-α/β activation of STAT4 is required for IFN-γ production.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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