Thermo Fisher Scientific CD183 (CXCR3) Monoclonal Antibody (CXCR3-173), NovaFluor Blue 660-120S, eBioscience

Thermo Fisher Scientific CD183 (CXCR3) Monoclonal Antibody (CXCR3-173), NovaFluor Blue 660-120S, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.6 µg/test
Publications: -

Product Specifications

Additional Formats

• APC
• Biotin
• Brilliant Violet 421
• Brilliant Violet 480
• FITC
• Functional Grade
• NovaFluor Blue 610-70S
• NovaFluor Blue 690
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436
• Super Bright 600
• Request custom conjugation

Product Specific Information

The monoclonal antibody CXCR3-173 recognizes mouse CD183 (CXCR3), a seven-transmembrane G-protein linked chemokine receptor binding CXCL9 (Mig), CXCL10 (IP-10), and CXCL11 (ITAC).
CD183 plays a role in CD4 T cell responses to grafts; knockout mice show compromised allograft rejection. Expression is found on NK cells, subsets of T lymphocytes and Tregs, and Th1-polarized cells. The antibody affects chemotaxis in response to ligand, and ligand presence eliminates staining. In vivo addition delays cardiac and pancreatic islet allograft rejection.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes. Recommended ≤ 0.25 µg/test (100 µL final volume). Optimize concentration empirically for best performance.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (PI, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
  • Add 5 µL per sample to antibody cocktails/master mixes before combining with cells.
  • For single-color controls, use 5 µL per 100 µL of cell sample (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology, providing engineered fluorescent signatures optimized for spillover and spread impacts.
Excitation: 509 nm
Emission: 665 nm
Laser: 488 nm (Blue)

Target Information

This gene encodes a G protein-coupled receptor selective for chemokines IP10, Mig, and I-TAC. These belong to the CXC chemokine family, inducing leukocyte traffic via integrin activation and chemotactic migration.
Inhibition by Bordetella pertussis toxin suggests coupling with Gi-class G protein. Responses are rapid and transient, restored after receptor recycling.
Prominently expressed in effector/memory T cells and inflamed tissues, contributing to inflammatory cell recruitment.
Target for small molecular antagonists in inflammatory disease research. Multiple transcript variants encoding different isoforms exist.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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