Thermo Fisher Scientific SOD2 Monoclonal Antibody (GT582)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:10,000

View 2 publications 2 publications

in situ PLA (PLA)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

GT582

Immunogen

Recombinant protein encompassing a sequence within the center region of human SOD2.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2787145

Product Specific Information

Keep as concentrated solution.

Predicted reactivity: Mouse (92%), Rat (94%), Zebrafish (88%), Xenopus laevis (86%), Pig (94%), Chicken (91%), Sheep (94%), Rhesus Monkey (98%), Chimpanzee (100%), Bovine (94%).

Positive Control: 293T, A431, HeLa, HepG2, mouse brain, rat brain.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Superoxide dismutase (SOD) is an antioxidant enzyme involved in the defense system against reactive oxygen species (ROS). SOD catalyzes the dismutation reaction of superoxide radical anion (O2) to hydrogen peroxide, which is then catalyzed to innocuous O2 and H2O by glutathione peroxidase and catalase. Several classes of SOD have been identified. These include intracellular copper, zinc SOD (Cu, Zn-SOD/SOD-1), mitochondrial manganese SOD (Mn-SOD/SOD-2) and extracellular Cu, Zn-SOD (EC-SOD/SOD-3). SOD1 is found in all eukaryotic species as a homodimeric 32 kDa enzyme containing one each of Cu and Zn ion per subunit. The manganese containing 80 kDa tetrameric enzyme SOD2, is located in the mitochondrial matrix in close proximity to a primary endogenous source of superoxide, the mitochondrial respiratory chain. SOD3 is a heparin-binding multimer of disulfide-linked dimers, primarily expressed in human lungs, vessel walls and airways. SOD4 is a copper chaperone for superoxide dismutase (CCS), which specifically delivers Cu to copper/zinc superoxide dismutase. CCS may activate copper/zinc superoxide dismutase through direct insertion of the Cu cofactor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.