Thermo Fisher Scientific beta-2 Microglobulin Monoclonal Antibody (9-E10-C6)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Flow Cytometry (Flow)

Assay-dependent

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species Reactivity

Reptile

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

9-E10-C6

Immunogen

Recombinant Caiman beta2-microglobulin.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1070705

Product Specific Information

KSK 003-01-02 reacts with free as well as MHC class I associated b2-microglobulin from the genus Caiman.

KSK 003-01-02 has been successfully used in immunohistochemistry and Western blotting techniques. KSK 003-01-02 is suitable for Western blotting, flow cytometry, immunoprecipitation, and affinity purification on caiman b2-microglobulin.

KSK 003-01-02 reacts with a non-polymorphic epitope on caiman b2-microglobulin.

NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL

Target Information

Beta-2-microglobulin (B2M) is a 12-14 kDa protein that is a subunit of the major histocompatibility complex (MHC) class I. Human beta 2 Microglobulin associated with HLA Class I antigens are expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells, but is absent on erythrocytes. Beta-2 microglobulin is also found in free form in the plasma. The amino acid sequence of Beta-2-microglobulin is homologous to the constant regions of immunoglobulin heavy and light chains, with a predominantly beta pleated sheet structure. The protein is ubiquitously expressed in the cytoplasm, and is involved in antigen processing and presentation. Beta-2-microglobulin also plays a role in TCR signaling, and downstream signaling in naive CD8+ cells. Beta 2-microglobulin is encoded by the B2M gene located on chromosome 15. A mutation in the Beta-2-microglobulin gene has been shown to result in hypercatabolic hypoproteinemia. Levels of beta 2 microglobulin can be used to assess renal function in kidney transplant recipients. Beta-2-microglobulin that protein can be used as a prognostic marker for HIV infection.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.