Invitrogen™

SuperScript™ Indirect cDNA Labeling System

The SuperScript™ Indirect cDNA Labeling System is an array labeling kit based on proven methods of indirect cDNA labeling. The자세히 알아보기

The SuperScript™ Indirect cDNA Labeling System is an array labeling kit based on proven methods of indirect cDNA labeling. The optimized system provides:

• SuperScript™ III RT for generating high cDNA yields
• A proprietary nucleotide mixture to increase signal intensity
• A convenient kit format that saves valuable time

In this indirect labeling method, mRNA or total RNA is reverse transcribed using SuperScript™ III RT, incorporating amino-modified dUTP and dATP into the synthesized cDNA. The template RNA is then degraded by base hydrolysis, and the reaction is neutralized with acid. The amino-modified cDNA is then purified to remove unincorporated nucleotides, primers, and buffers. In the second step, the modified cDNA is coupled with the active form of a fluorescent dye. The fluorescently labeled cDNA is purified with a S.N.A.P.™ spin column to remove any unreacted dye. The resulting fluorescently labeled cDNA is ready for hybridization to microarrays (Figure 1).

The SuperScript™ Indirect cDNA Labeling System provides a complete system that includes both the core kit reagents (except dyes) and a purification module. The SuperScript™ Indirect cDNA Labeling Module includes just the core kit reagents. Both provide the flexibility to use fluorescent nucleotides of your choice.

사양

제품 유형cDNA Labeling System

검출 방법Fluorescence

최종 제품 유형cDNA (Labeled)

라벨 또는 염료Alexa Fluor™ 555, Alexa Fluor™ 647, Cy™3, Cy™5

라벨 또는 염료 포함No

라벨링 타겟cDNA

라벨링 방법Indirect Labeling

샘플 종류RNA

제품라인SuperScript™

수량30 Reactions

Unit SizeEach