Thermo Fisher Scientific SuperScript Direct cDNA Labeling System

Invitrogen™ SuperScript™ Direct cDNA Labeling System

The SuperScript™ Direct cDNA Labeling System is a microarray labeling kit that combines the performance of SuperScript™ III Reverse Transcriptase (RT) with the simplicity of direct labeling to yield highly fluorescent labeled cDNA that accurately represents your initial mRNA sample.

In this method, anchored oligo(dT) anneals to the mRNA template. SuperScript™ III RT extends from the priming site, incorporating fluorescent dNTPs directly to produce labeled cDNA. Template RNA is degraded by base hydrolysis. Free nucleotides and other contaminants are removed using purification columns before hybridization to a microarray.

The SuperScript™ Direct cDNA Labeling System uses 800 units of SuperScript™ III RT to generate:

• High cDNA yields without an additional enzyme “spike”
• Strong signal intensities
• A fast and simple protocol that minimizes “hands-on” time and provides superior reproducibility

사양 (Specifications)

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