Thermo Fisher Scientific SuperScript Indirect cDNA Labeling Module, no purification columns

Invitrogen™ SuperScript™ Indirect cDNA Labeling Module (No Purification Columns)

The SuperScript™ Indirect cDNA Labeling System is an array labeling kit based on proven methods of indirect cDNA labeling.
This optimized system provides:

• SuperScript™ III RT for generating high cDNA yields
• A proprietary nucleotide mixture to increase signal intensity
• A convenient kit format that saves valuable time

Description

In this indirect labeling method, mRNA or total RNA is reverse transcribed using SuperScript™ III RT, incorporating amino-modified dUTP and dATP into the synthesized cDNA.
The template RNA is then degraded by base hydrolysis and neutralized with acid.
The amino-modified cDNA is purified to remove unincorporated nucleotides, primers, and buffers.

In the second step, the modified cDNA is coupled with the active form of a fluorescent dye.
The fluorescently labeled cDNA is purified using a S.N.A.P.™ spin column to remove any unreacted dye.
The resulting fluorescently labeled cDNA is ready for hybridization to microarrays.

The SuperScript™ Indirect cDNA Labeling System provides a complete system that includes both the core kit reagents (except dyes) and a purification module.
The SuperScript™ Indirect cDNA Labeling Module includes only the core kit reagents, offering flexibility to use fluorescent nucleotides of your choice.

Specifications

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