Thermo Fisher Scientific IL-28A Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

• In direct ELISAs and Western blots, approximately 60% cross-reactivity with human IL-28B/IFN-lambda 3 is observed.
• In direct ELISAs, approximately 30% cross-reactivity with recombinant human IL-29 and less than 5% cross-reactivity with recombinant mouse IL-28 is observed.
• Reconstitute at 0.2 mg/mL in sterile PBS.
• Endotoxin level is <0.10 EU per 1 µg of the antibody (LAL method).

Target Information

IL-28A belongs to the IFN lambda family, a novel family of cytokines within the IL-10 superfamily. The three members of this family are IL-29 (IFN lambda 1), IL-28A (IFN lambda 2), and IL-28B (IFN lambda 3), also known as type III interferons.
These cytokines signal through a heterodimeric receptor composed of IL-10R2 and IFN lambda R1 (IL-28R alpha). Signaling occurs via the Jak/STAT pathway, similar to type I interferons, activating antiviral and immunoregulatory genes.
Unlike type I IFNs, IFN lambda responses are limited to dendritic and certain tumor cells due to restricted receptor expression. IFN lambda stimulation can drive dendritic cells toward CD4+CD25+FoxP3+ regulatory T cell production, suggesting an immunoregulatory role.
Both IFN families exhibit antiviral, antiproliferative, and antitumor effects, making IFN lambdas potential alternatives to IFN alpha in cancer therapies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.