
Thermo Fisher Scientific POLR2A Polyclonal Antibody
POLR2A 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB, IHC, ICC, ELISA, ChIP 등 다양한 응용에 적합합니다. 인간, 생쥐, 랫트 반응성이 있으며 고순도 Affinity Chromatography 정제 제품입니다. 핵 내 POLR2A 검출에 유용합니다.
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Thermo Fisher Scientific POLR2A Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
| ChIP assay | 5 µg antibody for 10–15 µg of chromatin |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 1–260 of human POLR2A (NP_0009281) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.69 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2804643 |
Product Specific Information
- Positive Samples: HeLa, BT-474, H460, Jurkat, Mouse testis, Mouse brain
- Cellular Location: Nucleus
Immunogen Sequence:
MHGGGPPSGD SACPLRTIKR VQFGVLSPDE LKRMSVTEGG IKYPETTEGG RPKLGGLMDP RQGVIERTGR CQTCAGNMTE CPGHFGHIEL AKPVFHVGFL VKTMKVLRCV CFFCSKLLVD SNNPKIKDIL AKSKGQPKKR LTHVYDLCKG KNICEGGEEM DNKFGVEQPE GDEDLTKEKG HGGCGRYQPR IRRSGLELYA EWKHVNEDSQ EKKILLSPER VHEIFKRISD EECFVLGMEP RYARPEWMIV TVLPVPPLSV
Target Information
DNA-directed RNA polymerase II subunit RPB1 (POLR2A) is a DNA-dependent RNA polymerase that catalyzes transcription of DNA into RNA using ribonucleoside triphosphates as substrates. POLR2A is the largest subunit and forms the catalytic core of RNA polymerase II, responsible for synthesizing mRNA precursors and many non-coding RNAs. It participates in the active center together with the second largest subunit and plays a key role in transcription initiation, elongation, and termination. The phosphorylation status of its C-terminal domain (CTD) regulates interactions with transcription and mRNA processing factors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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